Coding
SAMsynth
Part:BBa_K417000:Design
Designed by: Ben Porebski, Andrew Perry Group: iGEM10_Monash_Australia (2010-10-14)
S-adenosyl methionine (SAM) synthetase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 102
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 682
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The genomic sequence corresponding to this part does not contain any illegal restriction sites with respect to RFC10, so no mutagenesis of the native sequence was required.
Source
Part was cloned from genomic DNA of Escherichia coli strain BL21(DE3) using PCR.
References
<biblio>
- Markham-1984 pmid=6094561
</biblio>
GenBank accession: [http://www.ncbi.nlm.nih.gov/nuccore/146838?from=86&to=1240&report=gbwithparts K02129.1]