Coding
SAMsynth

Part:BBa_K417000:Design

Designed by: Ben Porebski, Andrew Perry   Group: iGEM10_Monash_Australia   (2010-10-14)

S-adenosyl methionine (SAM) synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The genomic sequence corresponding to this part does not contain any illegal restriction sites with respect to RFC10, so no mutagenesis of the native sequence was required.


Source

Part was cloned from genomic DNA of Escherichia coli strain BL21(DE3) using PCR.

References

<biblio>

  1. Markham-1984 pmid=6094561

</biblio>

GenBank accession: [http://www.ncbi.nlm.nih.gov/nuccore/146838?from=86&to=1240&report=gbwithparts K02129.1]